Publications

  1. Wang, W., Dasetty, S., Sarupria, S., Blenner, M.* “Rational engineering of low temperature activity in thermoalkalophilic Geobacillus thermocatenulatus lipase”. Biochemical Engineering Journal 174:108093 (2021).

  2. Yaguchi, A., Lee, S., Blenner, M.* “Synthetic biology towards engineering microbial lignin biotransformation” Trends in Biotechnology (In Press, doi.org/10.1016/j.tibtech. 2021.02.003).  

  3. Yang, Z., Blenner, M.* “Genome editing systems across yeast” Current Opinion in Biotechnology 66:255-266 (2020).

  4. Czajka, J., Okumus, B., Blenner, M., Tang, Y.* “Mitigation of genetic drift by fluxome memory during microbial fermentation scale up” Current Opinion in Biotechnology 66:227-235 (2020).

  5. Molin, W., Yaguchi, A., Blenner, M., Saski, C. “Autonomous replication sequences from the Amaranthus palmeri eccDNA replicon enable replication in yeast” BMC Notes 13:330 (2020).

  6. Molin, W., Yaguchi, A., Blenner, M., Saski, C. “The eccDNA Replicon: A heritable, extra-nuclear vehicle that enables gene amplification and glyphosate resistance in Amaranthus palmeri” Plant Cell 32:2132-2140 (2020). 

  7. Yaguchi, A., Lee, S., Franaszek, N., O’Neill, K., Boundy-Mills, K., Blenner, M.*. “Identification of aromatic metabolizing oleaginous yeast”. Journal of Industrial Microbiology & Biotechnology 47:801-813 (2020). 

  8. Iqbal, S., Blenner, M., Alexander Bryant, A., Larsen, J. “Polymersomes for therapeutic delivery of macromolecules: From design to therapeutic applications”. Macromolecules 21:4 1327-1350 (2020).

  9. Ganesan, V. ‡, Spagnuolo, M. ‡, Agrawal, A., Smith, S., Gao, D., Blenner, M.* “Advances and opportunities in gene editing and gene regulation technology for Yarrowia lipolytica” Microbial Cell Factories 18:208 (2019).

  10. Schwartz, C., Cheng, JF., Evans, R., Schwartz, CA., Wagner, JM., Anglin, S., Beitz, A., Pan, W., Lonardi, S., Blenner, M., Alper, H., Yoshikuni, Y., Wheeldon, I.* “Validating genome-wide CRISPR-Cas9 function improves screening in the oleaginous yeast Yarrowia lipolytica” Metabolic Engineering 55:9 102-110 (2019).

  11. Spagnuolo, M., Yaguchi, A., Blenner, M.* “Engineering of oleaginous yeast for biofuels production: recent advances and future directions.” Current Opinion in Biotechnology 57:73-81 (2019).

  12. Gao, D., Smith, S., Rodriguez, G., Shabbir Hussain, M., Blenner, M.* “Dual CRISPR-Cas9 Cleavage in Yarrowia lipolytica Leads to Efficient Gene Excision and Targeted Integration”, Biotechnology Journal 13:9 1-8 (2018) doi: 10.1002/biot.201700590. 

  13. Spagnuolo, M., Shabbir Hussain M., Gambill L., Blenner, M.*, “Alternative Substrate Metabolism in Yarrowia lipolytica”, Frontiers in Microbiology 9:1077 (2018).

  14. Brabender, M., Shabbir Hussain, M., Rodriguez, G., Blenner, M.* “Urea and Urine are Efficient Nitrogen Sources for Growth of Yarrowia lipolytica”, Applied Microbiology and Biotechnology 102(5):2313-2322 (2018) DOI: 10.1007/s00253-018-8769-z

  15. Yaguchi, A., Spagnuolo, M., Blenner, M*. “Engineering Yeast for Utilization of Alternative Feedstocks” Current Opinion in Biotechnology 53:122-129 (2018).

  16. Yaguchi, A., Robinson A., Mihealsick E., Blenner, M.* “Metabolism of Aromatic Compounds by Trichosporon oleaginosus While Remaining Oleaginous”, Microbial Cell Factories 16:206 (2017) DOI: 10.1186/s12934-017-0820-8

  17. Dasetty, S., Blenner, M.*, Sarupria, S.* “Engineering Lipases: Walking the Fine Line Between Activity and Stability” Materials Research Express – Emerging Investigators Awards Series 4:114008 (2017) DOI: 10.1088/2053-1591/aa9946

  18. Shabbir Hussain, M., Wheeldon, I., Blenner, M.* “A Strong Hybrid Fatty Acid Inducible Transcriptional Sensor Built from Yarrowia lipolytica Upstream Activating and Regulatory Sequences”, Biotechnology Journal 12(1):1-11 (2017). DOI: 10.1002/biot.201700248. Research is featured on the inside cover.

  19. Bulutoglu, B., Dooley, K., Szilvay, G., Blenner M., and Banta, S.* “Catch & Release: Fishing for target proteins with an evolved β-roll peptide exhibiting allosteric regulation”, ACS Synthetic Biology 6(9):1732-1741 (2017). DOI: 10.1021/acssynbio.7b00089

  20. Yaguchi, A.‡, Rives, D.‡, Blenner, M.* “The New Kids on the Block: Emerging Oleaginous Yeast of Biotechnological Importance”, AIMS Microbiology 3(2):186-206 (2017). Top 4 most read in 2017.

  21. Schwartz, C., Shabbir-Hussain, M., Frogue, K., Blenner, M., Wheeldon, I.* “Standardized markerless gene integration for pathway engineering in Yarrowia lipolytica”, ACS Synthetic Biology 6(3):402-409 (2017). ISI Highly Cited.

  22. Shabbir Hussain, M.‡, Rodriguez, G.‡, Gao. D., Spagnuolo M., Gambill, L., Blenner, M.* “Recent Advances in Bioengineering of the Oleaginous Yeast Yarrowia lipolytica”, AIMS Bioengineering 3(4):493-514 (2016).

  23. Rodriguez, G.‡, Shabbir Hussain, M.‡, Gambill L., Gao, D., Yaguchi, A., Blenner, M.* “Engineering Xylose Utilization in Yarrowia lipolytica by Understanding its Cryptic Xylose Pathway”, Biotechnology for Biofuels 9:149 (2016).

  24. Schwartz, C., Shabbir-Hussain, M., Blenner, M., Wheeldon, I.* “Synthetic RNA Polymerase III Promoters Facilitate High Efficiency CRISPR-Cas9 Mediated Genome Editing in Yarrowia lipolytica”, ACS Synthetic Biology5(4):356-359. (2016). Paper is most read during past 12 months in 2016-2017. ISI Highly Cited.

  25. Shabbir Hussain, M., Gambill L., Smith, S., Blenner, M.* “Engineering Promoter Architecture in Oleaginous Yeast Yarrowia lipolytica”, ACS Synthetic Biology, 5(3):213-223. (2016). Research is featured on the cover of the March 2016 issue.

  26. Wilson, A., Blenner, M., Guiseppi-Elie, A.* “Polyplex Formation Influences Release Mechanism of Mono- and Di-Valent Ions from Phosphorylcholine Group Bearing Hydrogels”, Polymers, 6, 2451-2472 (2014).

  27. Nedumpully-Govindan, P., Lin, L., Alexov, E., Blenner, M., Ding, F*. “Structural and Energetic Determinants of Tyrosylprotein Sulfotransferase Specificity”. Bioinformatics, 15(30):2302-2309 (2014).

  28. Blenner, M., Dong, X., Springer, T*. “The structural basis of regulation of von Willebrand Factor binding to glycoprotein Ib”, J. Biological Chemistry, 289:5565-5579 (2014).

  29. Shur, O., Dooley, K., Blenner, M., Baltimore, M., Banta, S*. “A designed, phase changing RTX-based peptide for efficient bioseparations”, Biotechniques, 54(4):197-206 (2013). 

  30. Banta, S.*, Wheeldon, I., Blenner, M. “Protein engineering in the development of functional hydrogels” Annual Reviews in Biomedical Engineering, 12, 167-186 (2010)

  31. Blenner, M., Shur, O., Szilvay, G., Cropeck, D., Banta, S*. (2010), “Calcium induced folding of a Repeat in Toxin (RTX)- Domain Via C-Terminal Entropic Stabilization” Journal of Molecular Biology, 400, 244-256 (2010).

  32. Szilvay, G., Blenner, M., Cropeck, D., Banta, S*. “A FRET-based Method for Probing the Conformational Behavior of an Intrinsically Disordered Repeat Domain from Bordetella pertussis Adenylate Cyclase” Biochemistry, 48, 11273-11282 (2009).

  33. Blenner, M., Banta, S*. “Characterization of the 4D5Flu single chain antibody with a stimulus-responsive elastin-like peptide linker: A potential reporter of peptide linker conformation” Protein Science, 17, 527-536 (2008).

  34. Chockalingam, K., Blenner, M., Banta, S*. “Design and application of stimulus responsive peptide systems” Protein Engineering Design and Selection, 20, 155-61 (2007).

Books and Monographs

  1. Spagnuolo, M., Blenner, M.* “Gene excision by dual-guide CRISPR-Cas9” Methods in Molecular Biology,Yarrowia lipolytica, Vol 2307, Chapter 5, pg 85-94 (2021).

  2. Spagnuolo, M., Blenner, M.* “Simultaneous gene excision and integration with dual-guide CRISPR-Cas9”Methods in Molecular Biology, Yarrowia lipolytica, Vol 2307, Chapter 4, pg 69-83 (2021).

  3. Blenner, M.* "Enzymatic Deconstruction of Lignin for Biofuels" in Advances in Enzymatic Conversion of Biomass to Biofuels, 1st Edition (2015), Future Medicine Ltd, Montreal Canada.

 

Patents & Intellectual Property

  1. Banta, S*., Blenner, M., Wheeldon, I., Dooley, K. “Leucine Beta Roll Domains And Uses Thereof” US 9,127,267 B2 (September 2015)

Non-Refereed Journal Publications

Wang, W., Dasetty, S., Sarupria, S., Blenner, M.* “Rational engineering of low temperature activity in thermoalkalophilic Geobacillus thermocatenulatus lipase”. BioRxiv 

Molin, W., Yaguchi, A., Blenner, M., Saski, C. “The eccDNA Replicon: A heritable, extra-nuclear vehicle that enables gene amplification and glyphosate resistance in Amaranthus palmeri” BioRxiv https://doi.org/10.1101/2020.04.09.032896.

Blenner, M. “Microbes might be key to a mars mission” Scientific American Blog, 2019. https://blogs.scientificamerican.com/observations/microbes-might-be-key-to-a-mars-mission/

Blenner, M. “Influence of epigenetics on bioprocessing” , 9:72-76 (2018).

Schwartz, C., Cheng, JF., Evans, R., Schwartz, CA., Wagner, JM., Anglin, S., , Pan, W., Lonardi, S., Blenner, M., Alper, H., Yoshikuni, Y., Wheeldon, I.* “Validating genome-wide CRISPR-Cas9 function in the non-conventional yeast ”  (2018).