Publications
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Grissom, S., Dixon, Z., Singh, A., and Blenner, M.* “Heritable non-genetic phenotypes are enriched for stress responses as a form of bet hedging” BioRxiv (2024).
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Stohr, A., Ma, D., Chen, W.*, Blenner, M.* “Engineering conditional protein-protein interactions for dynamic cellular control” Biotechnology Advances 77:108457 (2024).
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Stepnov, A., Lopez Tavera, E., Klauer, R., Lincoln, C., Chowreddy, R., Beckham, G., Eijsink, V., Solomon, K., Blenner, M., Vaaje-Kolstad, G.* “Revisiting the activity of two recently reported plastic-acting enzymes” Nature Communications 15:8501 (2024).
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Rives, D., Peak, C., Blenner, M.* “RNASeq Analysis Highlights ATF6 Pathway Regulators for CHO Cell Engineering: Different Impacts of ATF6β and WFS1 Knockdown on Fed-batch Production of IgG1”. Scientific Reports 14:14141 (2024).
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Klauer, R., Hansen, A., Wu, D., Monteiro, L., Solomon, K. *, and Blenner, M.* “Biological upcycling of plastic waste” Annual Reviews in Chemical & Biomolecular Engineering 15:315-342 (2024).
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Brown, J.L.,Ç‚ Rodriguez-Ocasio,E.,Ç‚ Peterson,C.A.,Ç‚ Blenner, M.A., Smith,R.G., Jarboe, L.R.*, Brown, R.C.* “High-temperature noncatalytic oxidation of polyethylene to a fermentation substrate robustly utilized by Candida maltosa”. ACS Sustainable Chemistry & Engineering 11:50, 17778-17786 (2023).
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Agrawal, A.Ç‚, Yang, Z.Ç‚, Blenner, M.* “Metabolic Engineering of geraniol production in Yarrowia lipolytica” Metabolic Engineering Communications 17:e00228 (2023).
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Ganesan, V., Monteiro, L., Pedada, D., Stohr, A., Blenner, M* “High-efficiency multiplexed cytosine base editors for natural product biosynthesis in Yarrowia lipolytica” ACS Synthetic Biology 12:10 3082-3091 (2023).
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McCarthy, M.E., Dodd, W.B., Lu., X., Pritko, D., Patel, N., Haskel, C., Sanabria, H., Blenner, M., Birtwistle, M.R.* “A Theory for Non-Destructive, High-Throughput Genetic and Genetic Interaction Screening in Single Cells.” ACS Synthetic Biology 12:8 2290-2300 (2023).
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Wintenberg, M., Manglass, L., Martinez, N., Blenner, M.* “Global transcriptional response of Escherichia coli exposed in situ to different low-dose ionizing radiation sources” mSystems 8:2 (2023).
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Manglass, L., Wintenberg, M., Blenner, M. Martinez, N.*, “Flowthrough of Pu-239 and Fe-55 during RNA extraction” Journal of Radiological Protection 43:1 (2023).
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Wang, W., and Blenner, M.* “Engineering heterologous enzyme secretion in Yarrowia lipolytica”. Microbial Cell Factories 21:134 (2022).
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Rodriguez-Ocasio,E.,Ç‚ Khalid, A., Truka, C., Blenner, M.A., Jarboe, L.R.*, “Survey of non-conventional yeasts for lipid biotechnology”. Journal of Industrial Microbiology & Biotechnology 49:4 (2022).
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Ham, R., Smother, A., King, K., Napalitano, J., Swann, T., Pekarek, L., Blenner, M.*, Dean, D.*, “Efficient SARS-CoV-2 RT-qPCR Saliva Diagnostic Strategy Utilizing Open-Source Pipetting Robots” Journal of Visualized Experiments 180, e63395, doi:10.3791/63395 (2022).
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Manglass, L., Wintenberg, M., Vogel, C., Blenner, M., Martinez, N. "Accumulation of radio-iron and plutonium, alone and in combination, in Pseudomonas putida grown in liquid cultures" Journal of Radiological Protection 41(4):1199 (2021).
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Manglass, L., Wintenberg, M., Blenner, M., Martinez, N.* "Plutonium-239 accumulation in E. coli and P. putida grown in liquid cultures" Health Physics Journal doi: 10.1097 /HP0001455 (2021).
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Wang, W., Dasetty, S., Sarupria, S., Blenner, M.* “Rational engineering of low temperature activity in thermoalkalophilic Geobacillus thermocatenulatus lipase”. Biochemical Engineering Journal 174:108093 (2021).
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Yaguchi, A., Lee, S., Blenner, M.* “Synthetic biology towards engineering microbial lignin biotransformation” Trends in Biotechnology (In Press, doi.org/10.1016/j.tibtech. 2021.02.003).
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Yang, Z., Blenner, M.* “Genome editing systems across yeast” Current Opinion in Biotechnology 66:255-266 (2020).
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Czajka, J., Okumus, B., Blenner, M., Tang, Y.* “Mitigation of genetic drift by fluxome memory during microbial fermentation scale up” Current Opinion in Biotechnology 66:227-235 (2020).
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Molin, W., Yaguchi, A., Blenner, M., Saski, C. “Autonomous replication sequences from the Amaranthus palmeri eccDNA replicon enable replication in yeast” BMC Notes 13:330 (2020).
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Molin, W., Yaguchi, A., Blenner, M., Saski, C. “The eccDNA Replicon: A heritable, extra-nuclear vehicle that enables gene amplification and glyphosate resistance in Amaranthus palmeri” Plant Cell 32:2132-2140 (2020).
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Yaguchi, A., Lee, S., Franaszek, N., O’Neill, K., Boundy-Mills, K., Blenner, M.*. “Identification of aromatic metabolizing oleaginous yeast”. Journal of Industrial Microbiology & Biotechnology 47:801-813 (2020).
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Iqbal, S., Blenner, M., Alexander Bryant, A., Larsen, J. “Polymersomes for therapeutic delivery of macromolecules: From design to therapeutic applications”. Macromolecules 21:4 1327-1350 (2020).
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Ganesan, V. ‡, Spagnuolo, M. ‡, Agrawal, A., Smith, S., Gao, D., Blenner, M.* “Advances and opportunities in gene editing and gene regulation technology for Yarrowia lipolytica” Microbial Cell Factories 18:208 (2019).
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Schwartz, C., Cheng, JF., Evans, R., Schwartz, CA., Wagner, JM., Anglin, S., Beitz, A., Pan, W., Lonardi, S., Blenner, M., Alper, H., Yoshikuni, Y., Wheeldon, I.* “Validating genome-wide CRISPR-Cas9 function improves screening in the oleaginous yeast Yarrowia lipolytica” Metabolic Engineering 55:9 102-110 (2019).
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Spagnuolo, M., Yaguchi, A., Blenner, M.* “Engineering of oleaginous yeast for biofuels production: recent advances and future directions.” Current Opinion in Biotechnology 57:73-81 (2019).
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Gao, D., Smith, S., Rodriguez, G., Shabbir Hussain, M., Blenner, M.* “Dual CRISPR-Cas9 Cleavage in Yarrowia lipolytica Leads to Efficient Gene Excision and Targeted Integration”, Biotechnology Journal 13:9 1-8 (2018) doi: 10.1002/biot.201700590.
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Spagnuolo, M., Shabbir Hussain M., Gambill L., Blenner, M.*, “Alternative Substrate Metabolism in Yarrowia lipolytica”, Frontiers in Microbiology 9:1077 (2018).
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Brabender, M., Shabbir Hussain, M., Rodriguez, G., Blenner, M.* “Urea and Urine are Efficient Nitrogen Sources for Growth of Yarrowia lipolytica”, Applied Microbiology and Biotechnology 102(5):2313-2322 (2018) DOI: 10.1007/s00253-018-8769-z
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Yaguchi, A., Spagnuolo, M., Blenner, M*. “Engineering Yeast for Utilization of Alternative Feedstocks” Current Opinion in Biotechnology 53:122-129 (2018).
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Yaguchi, A., Robinson A., Mihealsick E., Blenner, M.* “Metabolism of Aromatic Compounds by Trichosporon oleaginosus While Remaining Oleaginous”, Microbial Cell Factories 16:206 (2017) DOI: 10.1186/s12934-017-0820-8
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Dasetty, S., Blenner, M.*, Sarupria, S.* “Engineering Lipases: Walking the Fine Line Between Activity and Stability” Materials Research Express – Emerging Investigators Awards Series 4:114008 (2017) DOI: 10.1088/2053-1591/aa9946
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Shabbir Hussain, M., Wheeldon, I., Blenner, M.* “A Strong Hybrid Fatty Acid Inducible Transcriptional Sensor Built from Yarrowia lipolytica Upstream Activating and Regulatory Sequences”, Biotechnology Journal 12(1):1-11 (2017). DOI: 10.1002/biot.201700248. Research is featured on the inside cover.
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Bulutoglu, B., Dooley, K., Szilvay, G., Blenner M., and Banta, S.* “Catch & Release: Fishing for target proteins with an evolved β-roll peptide exhibiting allosteric regulation”, ACS Synthetic Biology 6(9):1732-1741 (2017). DOI: 10.1021/acssynbio.7b00089
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Yaguchi, A.‡, Rives, D.‡, Blenner, M.* “The New Kids on the Block: Emerging Oleaginous Yeast of Biotechnological Importance”, AIMS Microbiology 3(2):186-206 (2017). Top 4 most read in 2017.
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Schwartz, C., Shabbir-Hussain, M., Frogue, K., Blenner, M., Wheeldon, I.* “Standardized markerless gene integration for pathway engineering in Yarrowia lipolytica”, ACS Synthetic Biology 6(3):402-409 (2017). ISI Highly Cited.
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Shabbir Hussain, M.‡, Rodriguez, G.‡, Gao. D., Spagnuolo M., Gambill, L., Blenner, M.* “Recent Advances in Bioengineering of the Oleaginous Yeast Yarrowia lipolytica”, AIMS Bioengineering 3(4):493-514 (2016).
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Rodriguez, G.‡, Shabbir Hussain, M.‡, Gambill L., Gao, D., Yaguchi, A., Blenner, M.* “Engineering Xylose Utilization in Yarrowia lipolytica by Understanding its Cryptic Xylose Pathway”, Biotechnology for Biofuels 9:149 (2016).
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Schwartz, C., Shabbir-Hussain, M., Blenner, M., Wheeldon, I.* “Synthetic RNA Polymerase III Promoters Facilitate High Efficiency CRISPR-Cas9 Mediated Genome Editing in Yarrowia lipolytica”, ACS Synthetic Biology5(4):356-359. (2016). Paper is most read during past 12 months in 2016-2017. ISI Highly Cited.
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Shabbir Hussain, M., Gambill L., Smith, S., Blenner, M.* “Engineering Promoter Architecture in Oleaginous Yeast Yarrowia lipolytica”, ACS Synthetic Biology, 5(3):213-223. (2016). Research is featured on the cover of the March 2016 issue.
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Wilson, A., Blenner, M., Guiseppi-Elie, A.* “Polyplex Formation Influences Release Mechanism of Mono- and Di-Valent Ions from Phosphorylcholine Group Bearing Hydrogels”, Polymers, 6, 2451-2472 (2014).
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Nedumpully-Govindan, P., Lin, L., Alexov, E., Blenner, M., Ding, F*. “Structural and Energetic Determinants of Tyrosylprotein Sulfotransferase Specificity”. Bioinformatics, 15(30):2302-2309 (2014).
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Blenner, M., Dong, X., Springer, T*. “The structural basis of regulation of von Willebrand Factor binding to glycoprotein Ib”, J. Biological Chemistry, 289:5565-5579 (2014).
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Shur, O., Dooley, K., Blenner, M., Baltimore, M., Banta, S*. “A designed, phase changing RTX-based peptide for efficient bioseparations”, Biotechniques, 54(4):197-206 (2013).
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Banta, S.*, Wheeldon, I., Blenner, M. “Protein engineering in the development of functional hydrogels” Annual Reviews in Biomedical Engineering, 12, 167-186 (2010)
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Blenner, M., Shur, O., Szilvay, G., Cropeck, D., Banta, S*. (2010), “Calcium induced folding of a Repeat in Toxin (RTX)- Domain Via C-Terminal Entropic Stabilization” Journal of Molecular Biology, 400, 244-256 (2010).
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Szilvay, G., Blenner, M., Cropeck, D., Banta, S*. “A FRET-based Method for Probing the Conformational Behavior of an Intrinsically Disordered Repeat Domain from Bordetella pertussis Adenylate Cyclase” Biochemistry, 48, 11273-11282 (2009).
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Blenner, M., Banta, S*. “Characterization of the 4D5Flu single chain antibody with a stimulus-responsive elastin-like peptide linker: A potential reporter of peptide linker conformation” Protein Science, 17, 527-536 (2008).
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Chockalingam, K., Blenner, M., Banta, S*. “Design and application of stimulus responsive peptide systems” Protein Engineering Design and Selection, 20, 155-61 (2007).
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Books and Monographs
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Spagnuolo, M., Blenner, M.* “Gene excision by dual-guide CRISPR-Cas9” Methods in Molecular Biology,Yarrowia lipolytica, Vol 2307, Chapter 5, pg 85-94 (2021).
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Spagnuolo, M., Blenner, M.* “Simultaneous gene excision and integration with dual-guide CRISPR-Cas9”Methods in Molecular Biology, Yarrowia lipolytica, Vol 2307, Chapter 4, pg 69-83 (2021).
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Blenner, M.* "Enzymatic Deconstruction of Lignin for Biofuels" in Advances in Enzymatic Conversion of Biomass to Biofuels, 1st Edition (2015), Future Medicine Ltd, Montreal Canada.
Patents & Intellectual Property
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Yoon, S., Fu, Q., Blenner, M., Doleh, E. “Inducible stable packaging cell line for rAAV production” US 63/662,862 (Patent Application filed June 21, 2024).
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Blenner, M., Solomon, K., Klauer, R. “Biological and Biomolecular Recovery of Plastic Waste” US 63/564,161 (Patent Application filed March 12, 2024).
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Blenner, M., Sivachandaran, F., Spagnuolo, M. “Microbial Production of Fatty Alcohols Via Spatial Enzyme Localization in the Peroxisomes of Yarrowia lipolytica” US 63/392,900 (Provisional Application- July 28, 2022).
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Blenner, M., Solomon, K., Monteiro, L., Singh, J., Klauer, R. “Optimizing Plastic Degradation by Yellow Mealworms and Their Gut Microbiomes” US 63/336,657 (Patent Application filed April 28, 2022, PCT/US2023/066382).
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Banta, S*., Blenner, M., Wheeldon, I., Dooley, K. “Leucine Beta Roll Domains And Uses Thereof” US 9,127,267 B2 (September 2015)
Non-Refereed Journal Publications
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Klauer, R., Blenner, M., Solomon, K.* “”Life… finds a way”: Sustainable capture and upcycling of plastics by microbes” National Academy of Engineering Quarterly, 2022.
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Blenner, M. “Microbes might be key to a mars mission” Scientific American Blog, 2019.
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Blenner, M. “Influence of epigenetics on bioprocessing” , 9:72-76 (2018).